	US 12,252,693 B2
	Plasmids for manipulation of Wolbachia
Sarah Bordenstein, Nashville, TN (US); Julie Reveillaud, Montpellier (FR); A. Murat Eren, Chicago, IL (US); and Seth Bordenstein, Nashville, TN (US)
Assigned to VANDERBILT UNIVERSITY, Nashville, TN (US); and THE UNIVERSITY OF CHICAGO, Chicago, IL (US)
Appl. No. 17/293,634
Filed by VANDERBILT UNIVERSITY, Nashville, TN (US); INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT, Paris (FR); and THE UNIVERSITY OF CHICAGO, Chicago, IL (US)
PCT Filed Nov. 15, 2019, PCT No. PCT/US2019/061611 § 371(c)(1), (2) Date May 13, 2021, PCT Pub. No. WO2020/102626, PCT Pub. Date May 22, 2020.
Claims priority of provisional application 62/768,165, filed on Nov. 16, 2018.
Claims priority of provisional application 62/811,131, filed on Feb. 27, 2019.
Prior Publication US 2022/0010317 A1, Jan. 13, 2022
Int. Cl. C12N 15/74 (2006.01)

CPC C12N 15/74 (2013.01) [C12N 2800/101 (2013.01)]

18 Claims

1. A non-naturally occurring plasmid comprising a first nucleic acid sequence capable of transforming a Wolbachia cell, wherein the first nucleic acid sequence comprises a transposase encoded by a nucleotide sequence at least 90% identical to SEQ ID NO: 1.