US 12,252,687 B2
Recombinant yeast and use thereof
Xueli Zhang, Tianjin (CN); Luqi Huang, Beijing (CN); Zhubo Dai, Tianjin (CN); Dong Wang, Tianjin (CN); Lili Zhang, Tianjin (CN); Juan Guo, Beijing (CN); and Yi Liu, Tianjin (CN)
Assigned to Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin (CN); and Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing (CN)
Filed by Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin (CN); and Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing (CN)
Filed on Aug. 25, 2023, as Appl. No. 18/456,333.
Application 18/456,333 is a continuation of application No. 17/812,157, filed on Jul. 12, 2022, abandoned.
Application 17/812,157 is a continuation of application No. 16/347,552, granted, now 11,421,199, issued on Aug. 23, 2022, previously published as PCT/CN2017/109029, filed on Nov. 2, 2017.
Claims priority of application No. 201610961269.5 (CN), filed on Nov. 4, 2016.
Prior Publication US 2024/0010969 A1, Jan. 11, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 1/18 (2006.01); C12N 9/10 (2006.01); C12N 9/88 (2006.01); C12R 1/865 (2006.01)
CPC C12N 1/18 (2013.01) [C12N 1/185 (2021.05); C12N 9/1085 (2013.01); C12N 9/88 (2013.01); C12Y 205/0101 (2013.01); C12Y 402/03023 (2013.01); C12N 2500/05 (2013.01); C12N 2500/10 (2013.01); C12N 2500/32 (2013.01); C12N 2500/34 (2013.01); C12N 2500/38 (2013.01); C12R 2001/865 (2021.05)] 25 Claims
 
1. A recombinant yeast strain, comprising:
a fusion protein
comprising germacrene A synthetase and farnesyl pyrophosphate synthase, wherein the fusion protein is one or more selected from the group consisting of:
(1) SynSmFPS-GGGS (SEQ ID NO: 15)-STpGMAS,
(2) SynSmFPS-PGGH (SEQ ID NO: 16)-STpGMAS,
(3) SynSmFPS-YRSQI (SEQ ID NO: 17)-STpGMAS,
(4) SynSmFPS-VIPFIS (SEQ ID NO: 18)-STpGMAS,
(5) SynSmFPS-FLYLKF (SEQ ID NO: 19)-STpGMAS,
(6) SynSmFPS-WRFSPKLQ (SEQ ID NO: 20)-STpGMAS,
(7) SynSmFPS-HHVQESQCISTV (SEQ ID NO: 21)-STpGMAS, and
(8) a combination of SynSmFPS-WRFSPKLQ (SEQ ID NO: 20)-STpGMAS, ERG20-GGGS (SEQ ID NO: 15)-LsLTSC2 and SynSmFPS-GGGS (SEQ ID NO: 15)-STpGMAS.