US 12,252,684 B2
Device and method for controlling a bioreactor
Ohad Karnieli, Kiryat Tivon (IL); and Noam Bercovich, Haifa (IL)
Assigned to ADVA Biotechnology Ltd., Kiryat Tivon (IL)
Filed by ADVA Biotechnology Ltd., Kiryat Tivon (IL)
Filed on Apr. 4, 2023, as Appl. No. 18/295,384.
Application 18/295,384 is a continuation of application No. 17/688,115, filed on Mar. 7, 2022, granted, now 11,667,882.
Application 17/688,115 is a continuation of application No. 17/153,062, filed on Jan. 20, 2021, abandoned.
Claims priority of provisional application 62/963,306, filed on Jan. 20, 2020.
Prior Publication US 2023/0383236 A1, Nov. 30, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12M 1/36 (2006.01); C12M 1/00 (2006.01); C12M 1/34 (2006.01); C12Q 1/00 (2006.01); C12Q 1/02 (2006.01)
CPC C12M 41/48 (2013.01) [C12M 29/10 (2013.01); C12M 41/12 (2013.01); C12M 41/26 (2013.01); C12M 41/32 (2013.01); C12M 41/34 (2013.01); C12M 41/36 (2013.01); C12M 41/44 (2013.01); C12Q 1/00 (2013.01); C12Q 1/02 (2013.01)] 20 Claims
 
1. A bioreactor system comprising:
a bioreactor chamber,
wherein the bioreactor chamber contains grown cells;
at least one sensor in the bioreactor chamber,
wherein the at least one sensor is configured to determine actual values of three parameters within the bioreactor chamber, the three parameters comprising:
a nutrient level within the bioreactor chamber,
a pH level within the bioreactor chamber, and
an O2 level within the bioreactor chamber,
a reservoir,
wherein the reservoir does not contain any cells;
a first fluid circuit configured to permit flow of a first fluid from the bioreactor chamber to the reservoir;
a second fluid circuit configured to permit flow of a second fluid from the reservoir to the bioreactor chamber;
a controller configured to flow
a first liquid from the bioreactor chamber into the reservoir through the first fluid circuit, and
a second liquid from the reservoir into the bioreactor chamber through the second fluid circuit to adjust simultaneously each of the actual values of the parameters within the bioreactor chamber to match a corresponding set point value of the parameter, after determining that each of the set point values within the bioreactor chamber is not equal to the corresponding actual value; and
a third fluid circuit configured to permit one-way flow of a waste product out of the bioreactor chamber,
wherein the first fluid circuit, the second fluid circuit, and the third fluid circuit are fluidly isolated from one another.