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1. An automated cell culture system comprising: an insertable and removable bioreactor comprising a plurality of cell culture compartments and formed from a plurality of stacked layers including a middle cell culture compartment, the bioreactor having a fluid port fluidly connected to the plurality of cell culture compartments; a pump fluidly connected to the fluid port for providing a cell culture medium to the plurality of culture compartments; a sensor operably connected to at least the middle cell culture compartment for measuring at least one cell culture parameter; one or more actuators operably connected to the middle cell culture compartment configured to automatically control one or more cell culture parameters in the middle cell culture compartment based on an output from the sensor; and a controller electronically connected to the pump, the actuator, and the sensor, wherein the controller is configured to automatically adjust a pump flow rate, a cell culture gas content with a gas exchanger to provide a desired gas composition comprising an appropriate level of each of carbon dioxide, oxygen and nitrogen, and a cell culture parameter to provide an automated desired steady-state cell culture parameter to achieve ideal or desired cell culture conditions based on the output from the sensor for facilitating automated monitoring of cell growth and functional maintenance, cell communication and collection of products from cell interactions; wherein the insertable and removable bioreactor, pump, sensor, gas exchanger and one or more actuators and controller are modularly connected to provide an integrated and automated modular workstation having the insertable and removable bioreactor integrated into the automated modular workstation for tissue co-culture and configured for subsequent removal and shipment to a remote testing facility; wherein a lid and a base plate are positioned to contain the plurality of stacked layers; a fastener to reliably secure the lid top and base plate and fluidly enclose the bioreactor, wherein the plurality of stacked layers are formed of a compressible material; wherein the fastener comprises a clamp that compresses the plurality of stacked layers upon a clamp actuation; and the lid top and base plates have complimentary relief and recess features configured to provide a uniform force distribution over the plurality of stacked layers and provide a fluid seal with the clamp actuation.
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