| CPC C12M 41/44 (2013.01) [C12M 23/58 (2013.01); C12M 29/00 (2013.01); C12M 33/14 (2013.01)] | 19 Claims |

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1. A bioprocessing system, comprising:
a first fluid assembly configured for connection to one or more sources of fluid for input into the bioprocessing system, the first fluid assembly having a first fluid assembly line connected to a first port of a first bioreactor vessel though a first bioreactor line of the first bioreactor vessel, the first bioreactor line of the first bioreactor vessel including a first bioreactor line valve for providing selective fluid communication between the first fluid assembly and the first port of the first bioreactor vessel;
a second fluid assembly having a second fluid assembly line connected to a second port of the first bioreactor vessel through a second bioreactor line of the first bioreactor vessel, the second bioreactor line of the first bioreactor vessel including a second bioreactor line valve for providing selective fluid communication between the second fluid assembly and the second port of the first bioreactor vessel;
an interconnect line providing for fluid communication between the first fluid assembly and the second fluid assembly, and for fluid communication between the second bioreactor line of the first bioreactor vessel and the first bioreactor line of the first bioreactor vessel; and
a sampling assembly connected to at least one of the first fluid assembly, the second fluid assembly, the interconnect line and/or a fluid flowpath intermediate the second bioreactor line valve and the first bioreactor line valve, the sampling assembly including a sampling assembly line providing for fluid communication between the sampling assembly and the second port of the first bioreactor vessel;
wherein the sampling assembly is connected to the interconnect line intermediate the second bioreactor line valve and the first bioreactor line valve.
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