US 12,252,681 B2
Systems, devices, and methods for sterilizing bioreactors and culture media
Matthew Leung, Richmond, CA (US); Patricia Rae Benton, Huntington Beach, CA (US); Cameron Gentry Copeland, Walnut Creek, CA (US); Stephen K. Hsu, Albany, NY (US); and Kalle Lukyan Johnson, Orinda, CA (US)
Assigned to Upside Foods, Inc., Berkeley, CA (US)
Filed by Upside Foods, Inc., Berkeley, CA (US)
Filed on Aug. 18, 2020, as Appl. No. 16/996,567.
Prior Publication US 2022/0056394 A1, Feb. 24, 2022
Int. Cl. A61L 2/20 (2006.01); A61L 2/00 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12M 1/34 (2006.01); C12M 3/04 (2006.01)
CPC C12M 37/00 (2013.01) [A61L 2/0094 (2013.01); A61L 2/20 (2013.01); C12M 23/22 (2013.01); C12M 27/10 (2013.01); C12M 39/00 (2013.01); C12M 41/44 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of preparing cell culture media for growing cell-based products, the method comprising:
sterilizing an enclosed vessel and a hydrated cell culture media disposed therein in preparation to grow mammalian, avian, or aquatic animal cells by:
circulating a chlorine dioxide gas in the enclosed vessel to sterilize the hydrated cell culture media and the enclosed vessel; and
agitating the hydrated cell culture media while circulating the chlorine dioxide gas in the enclosed vessel for a dwell time sufficient to sterilize the hydrated cell culture media and the enclosed vessel.