US 12,252,680 B2
Bioreactor with filter unit and method for treating a cell broth
Martin Leuthold, Goettingen (DE); Alexander Helling, Ebergoetzen (DE); and Ulrich Grummert, Bad Sooden-Allendorf (DE)
Assigned to SARTORIUS STEDIM BIOTECH GMBH, Goettingen (DE)
Filed by Sartorius Stedim Biotech GmbH, Goettingen (DE)
Filed on Feb. 5, 2021, as Appl. No. 17/168,556.
Application 17/168,556 is a continuation of application No. PCT/EP2019/071199, filed on Aug. 7, 2019.
Claims priority of application No. 10 2018 006 286.5 (DE), filed on Aug. 8, 2018.
Prior Publication US 2021/0155888 A1, May 27, 2021
Int. Cl. C12M 1/00 (2006.01); B01D 61/14 (2006.01)
CPC C12M 29/04 (2013.01) [B01D 61/147 (2013.01); C12M 29/10 (2013.01); C12M 47/10 (2013.01); B01D 2319/025 (2013.01)] 12 Claims
OG exemplary drawing
 
1. Bioreactor with a filter unit, wherein
the bioreactor defines an interior, and
the filter unit comprises
at least one supply channel,
at least one first filter medium,
at least one retentate channel,
at least one second filter medium,
at least one permeate channel,
a further second filter medium,
a further retentate channel, and
a further first filter medium,
arranged such that
the first filter medium delimits the supply channel and the retentate channel from one another,
the second filter medium delimits the retentate channel and the permeate channel from one another,
the further second filter medium delimits the permeate channel and the further retentate channel from one another, and
the further first filter medium delimits the further retentate channel,
wherein
the supply channel is connected in a fluid conducting manner to at least one inlet for a supply medium and is connected in a fluid permeable manner via the first filter medium to the retentate channel,
the retentate channel is connected in a fluid conducting manner to at least one inlet for a cell broth and to at least one outlet for the cell broth and is connected in a fluid permeable manner via the second filter medium to the permeate channel,
the permeate channel is connected in a fluid conducting manner to at least one outlet for a permeate,
the interior of the bioreactor is connected in a fluid conducting manner to the inlet for the cell broth and in a fluid conducting manner to the outlet for the cell broth, and
the supply channel, the one first filter medium, the one retentate channel, the one second filter medium, the further second filter medium, the further retentate channel, and the further first filter medium form a stacked arrangement forming a filter cartridge.