US 12,252,679 B2
Method of using a bioreactor
Ohad Karnieli, Kiryat Tivon (IL)
Assigned to ADVA Biotechnology Ltd., Kiryat Tivon (IL)
Filed by ADVA Biotechnology Ltd., Kiryat Tivon (IL)
Filed on Dec. 18, 2023, as Appl. No. 18/543,007.
Application 18/543,007 is a continuation of application No. 18/049,110, filed on Oct. 24, 2022, granted, now 11,859,163.
Application 18/049,110 is a continuation of application No. 16/325,389, granted, now 11,549,090, issued on Jan. 10, 2023, previously published as PCT/IL2017/050927, filed on Aug. 21, 2017.
Claims priority of provisional application 62/489,065, filed on Apr. 24, 2017.
Claims priority of provisional application 62/377,628, filed on Aug. 21, 2016.
Prior Publication US 2024/0174958 A1, May 30, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12M 1/00 (2006.01); C12M 1/34 (2006.01); C12M 1/36 (2006.01)
CPC C12M 23/34 (2013.01) [C12M 29/14 (2013.01); C12M 29/18 (2013.01); C12M 41/12 (2013.01); C12M 41/40 (2013.01); C12M 41/42 (2013.01); C12M 41/48 (2013.01); C12M 21/00 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of growing cells, comprising:
obtaining a bioreactor,
wherein the bioreactor comprises a vessel,
wherein the vessel comprises a first chamber and a second chamber upstream of and in fluid communication with the first chamber;
introducing a plurality of cells into the second chamber;
introducing a fluid into the first chamber,
wherein the fluid comprises a nutrient;
flowing the fluid in a first direction, from the first chamber toward the second chamber, through a first plurality of pores, the first plurality of pores aligning the fluid, controlling a velocity of the fluid, and preventing passage of a bubble through the first plurality of pores, to thereby provide an aligned fluid;
flowing the aligned fluid in the first direction through a second plurality of pores into the second chamber, the second plurality of pores above the first plurality of pores, the second plurality of pores preventing the plurality of cells in the second chamber from passing from the second chamber into the first chamber;
decreasing the velocity of the aligned fluid as the fluid flows from a bottom end of the second chamber toward a top end of the second chamber, to thereby provide a reduced velocity fluid;
suspending at least some cells of the plurality of cells in the reduced velocity fluid, to thereby provide suspended cells;
growing the suspended cells with the nutrient, to thereby provide grown cells; and
removing at least a portion of the reduced velocity fluid from the second chamber, to thereby provide a removed fluid.