US 12,252,678 B2
Systems and methods for bioprocessing
Cesare M. Cejas, Edgware (GB); Antonio de Grazia, St. Albans (GB); Manjari Ghanshyam, Hatfield (GB); James Kusena, Hempstead (GB); and Sreedhar Mareddy, Chelmsford (GB)
Assigned to MicrofluidX Ltd, Stevenage (GB)
Filed by MicrofluidX Ltd, Stevenage (GB)
Filed on Sep. 26, 2022, as Appl. No. 17/953,016.
Claims priority of provisional application 63/285,062, filed on Dec. 1, 2021.
Prior Publication US 2023/0167392 A1, Jun. 1, 2023
Int. Cl. B01L 3/00 (2006.01); C12M 1/00 (2006.01); C12M 1/32 (2006.01); C12M 3/06 (2006.01)
CPC C12M 23/16 (2013.01) [B01L 3/502715 (2013.01); B01L 3/50273 (2013.01); B01L 3/502761 (2013.01); C12M 23/12 (2013.01); C12M 29/04 (2013.01); C12M 29/10 (2013.01); B01L 2400/0487 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A solid support comprising:
a bioprocessing chamber comprising a first bottom surface for culturing cells and a first ceiling opposite of the first bottom surface, wherein the first bottom surface and the first ceiling are connected by one or more first sidewalls, wherein the bioprocessing chamber comprises a first height measured between the first bottom surface and the first ceiling;
a collection output fluidically connected to the bioprocessing chamber via the first ceiling or the first bottom surface of the bioprocessing chamber;
a feeding input channel configured to receive a fluid, wherein the feeding input channel is fluidically connected to the bioprocessing chamber, wherein the feeding input channel comprises a second bottom surface and a second ceiling opposite of the second bottom surface, wherein the second bottom surface and the second ceiling are connected by one or more second sidewalls, wherein the feeding input channel comprises a second height measured between the second bottom surface and the second ceiling of the feeding input channel, wherein the first bottom surface and the second bottom surface are parallel, and wherein the second height is less than the first height;
a feeding output channel fluidically connected to the bioprocessing chamber via the first ceiling; and
a filter connected to the feeding output channel or the bioprocessing chamber, wherein the filter is configured to selectively prevent a passage of the at least one cell from the bioprocessing chamber to the feeding output channel.