US 12,252,677 B2
Fluidic device, fluidic system, and method for developing three-dimensional cellular constructions
Ulrike Nuber, Mühltal (DE); Heinz Koeppl, Darmstadt (DE); Steffen Hardt, Gross-Zimmern (DE); and Katrin Töpfer, Darmstadt (DE)
Assigned to TECHNISCHE UNIVERSITÄT DARMSTADT, Darmstadt (DE)
Appl. No. 17/287,749
Filed by TECHNISCHE UNIVERSITÄT DARMSTADT, Darmstadt (DE)
PCT Filed Oct. 28, 2019, PCT No. PCT/EP2019/079418
§ 371(c)(1), (2) Date Apr. 22, 2021,
PCT Pub. No. WO2020/089178, PCT Pub. Date May 7, 2020.
Claims priority of application No. 102018127406.8 (DE), filed on Nov. 2, 2018.
Prior Publication US 2021/0355422 A1, Nov. 18, 2021
Int. Cl. C12M 3/06 (2006.01); C12M 1/00 (2006.01); C12M 3/00 (2006.01)
CPC C12M 23/16 (2013.01) [C12M 21/08 (2013.01); C12M 23/34 (2013.01); C12M 27/22 (2013.01); C12M 29/00 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A fluidic device for developing a cellular starting material into a three-dimensional cellular structure, comprising a base body, including
a chamber extending in an x-direction, a y-direction orthogonal to the x-direction and a z-direction orthogonal to the x-direction and the y-direction, in which a matrix is received, into which the cellular starting material to be developed can be is introduced; and
at least two fluid reservoirs, wherein each of the at least two fluid reservoirs comprises:
a fluid inlet for introducing a fluid medium into the fluid reservoir;
a fluid outlet for discharging the fluid medium from the fluid reservoir; and
a separating device which is partially permeable to the fluid medium and which separates one of the at least two fluid reservoirs from the chamber and forms a common plane interface between the one of the at least two fluid reservoirs and the chamber, via which the fluid medium diffuses into the matrix;
wherein the fluidic device is adapted, using fluid media, to form at least one concentration gradient, at least two mutually orthogonal concentration gradients and/or at least two mutually antiparallel concentration gradients (X1, X2, Y1, Y2) in the matrix which are each essentially homogeneous or deliberately inhomogeneous over at least a section of the extension of the matrix in the z-direction, the adaptation being effected by
(i) two of the separating devices are present, with each extending in the z-direction and the x-direction and being arranged opposite to one another and being spaced from one another by the chamber in the y-direction; and/or
(ii) two more of the separating devices are present, with each extending in the z-direction and the y-direction and being arranged opposite to one another and being spaced from one another by the chamber in the x-direction.