US 12,252,676 B2
Cell culture system
Osamu Koseki, Kanagawa (JP); and Satoshi Tanaka, Kanagawa (JP)
Assigned to TOYO SEIKAN GROUP HOLDINGS, LTD., Tokyo (JP)
Filed by TOYO SEIKAN GROUP HOLDINGS, LTD., Tokyo (JP)
Filed on Oct. 25, 2021, as Appl. No. 17/509,119.
Application 17/509,119 is a continuation of application No. PCT/JP2020/008608, filed on Mar. 2, 2020.
Claims priority of application No. 2019-086904 (JP), filed on Apr. 27, 2019.
Prior Publication US 2022/0041965 A1, Feb. 10, 2022
Int. Cl. C12M 3/00 (2006.01); C12M 1/00 (2006.01); C12M 1/34 (2006.01); C12M 1/36 (2006.01)
CPC C12M 23/14 (2013.01) [C12M 23/26 (2013.01); C12M 23/48 (2013.01); C12M 29/14 (2013.01); C12M 41/48 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A cell culture system comprising:
a culture bag made of a flexible packaging material and having first and second ports;
a medium container that stores a medium to be transferred to the culture bag;
a controller that includes a processor, the controller being configured to control a supply of the medium;
a weight sensor or a length measurement sensor that measures a weight or length change, respectively, occurring in response to a change in amount of medium in the culture bag,
first and second tubular members;
first and second pumps;
wherein:
the first and second tubular members are connected to the first and second ports, respectively, to circularly connect the culture bag and the medium container through the first and second tubular members;
the first tubular member connects between the first port and the medium container, and the first pump is disposed in the first tubular member;
the second tubular member connects between the second port and the medium container, and the second pump is disposed in the second tubular member;
the controller is configured to:
calculate a liquid thickness of the medium in the culture bag based on detection information input from the weight sensor or the length measurement sensor, and,
based on the liquid thickness of the medium, control an operation of the first pump and the second pump to circulate the medium in the culture bag and the medium container, thereby making the liquid thickness in the culture bag within a certain range.