US 12,252,555 B2
Selenium-chelating pea oligopeptide, and preparation method and use thereof
Muyi Cai, Beijing (CN); Ruizeng Gu, Beijing (CN); Jun Lu, Beijing (CN); Wenying Liu, Beijing (CN); Xiuyuan Qin, Beijing (CN); Xingchang Pan, Beijing (CN); Zhe Dong, Beijing (CN); Yong Ma, Beijing (CN); Yaguang Xu, Beijing (CN); Yongqing Ma, Beijing (CN); Liang Chen, Beijing (CN); Lu Lu, Beijing (CN); Haixin Zhang, Beijing (CN); Ying Wei, Beijing (CN); Yan Liu, Beijing (CN); Kelu Cao, Beijing (CN); Jing Wang, Beijing (CN); Guoming Li, Beijing (CN); Ming Zhou, Beijing (CN); Yuchen Wang, Beijing (CN); Yuqing Wang, Beijing (CN); Kong Ling, Beijing (CN); Yuan Bi, Beijing (CN); and Xinyue Cui, Beijing (CN)
Assigned to CHINA NATIONAL RESEARCH INSTITUTE OF FOOD & FERMENTATION INDUSTRIES CO., LTD., Beijing (CN)
Filed by CHINA NATIONAL RESEARCH INSTITUTE OF FOOD & FERMENTATION INDUSTRIES CO., LTD., Beijing (CN)
Filed on Apr. 9, 2021, as Appl. No. 17/227,206.
Application 17/227,206 is a continuation of application No. PCT/CN2018/121723, filed on Dec. 18, 2018.
Claims priority of application No. 201811189889.7 (CN), filed on Oct. 12, 2018.
Prior Publication US 2021/0230225 A1, Jul. 29, 2021
Int. Cl. C07K 7/06 (2006.01)
CPC C07K 7/06 (2013.01) 7 Claims
 
1. A selenium-pea oligopeptide chelate, wherein the selenium-pea oligopeptide chelate contains an amino acid sequence of SEQ ID NO:2, and a mass content of SEQ ID NO:2 is not less than 25 ng/mg; acid soluble protein content of the selenium-pea oligopeptide chelate is more than 23%, total nitrogen content is more than 23%; the selenium-pea oligopeptide chelate is composition of selenium-pea oligopeptide chelate with different molecular weight, and a portion of selenium-pea oligopeptide chelate with molecular weight less than 1000 u accounts for more than 85% of a total amount of the selenium-pea oligopeptide chelate; and selenium content is not less than 0.08 g per 100 g of the selenium-pea oligopeptide chelate;
wherein the selenium-pea oligopeptide chelate is a reaction product of an aqueous solution of pea oligopeptide and sodium selenite, and selenium of the sodium selenite is stably combined with the pea oligopeptide in a way of covalent bond so that after the selenium-pea oligopeptide chelate is prepared and then subjected to digestion treatment in at least one of following three ways, a change rate of the selenium content is not more than 3% with respect to the selenium content before the digestion treatment:
hydrolyzing for 4 hours by a pepsin at a pH value of 2 and a temperature of 37° C.;
hydrolyzing for 6 hours by a trypsin at a pH value of 7.5 and a temperature of 37° C.;
maintaining a temperature constant at 37° C., and firstly hydrolyzing for 4 hours by a pepsin at a pH value of 2, and then continuing to hydrolyze for 6 hours by a trypsin at a pH value of 6.8;
wherein the change rate of the selenium content is a mass percentage of a mass of selenium decomposed during the digestion treatment of the selenium-pea oligopeptide chelate to a total mass of selenium in the selenium-pea oligopeptide chelate before the digestion treatment.