US 12,252,538 B2
Monoclonal antibodies against IgV domain of B7-H3 and uses thereof
Xingxing Zang, New York, NY (US)
Assigned to ALBERT EINSTEIN COLLEGE OF MEDICINE, Bronx, NY (US)
Appl. No. 17/294,560
Filed by Albert Einstein College of Medicine, Bronx, NY (US)
PCT Filed Nov. 15, 2019, PCT No. PCT/US2019/061887
§ 371(c)(1), (2) Date May 17, 2021,
PCT Pub. No. WO2020/102779, PCT Pub. Date May 22, 2020.
Claims priority of provisional application 62/768,128, filed on Nov. 16, 2018.
Prior Publication US 2022/0010018 A1, Jan. 13, 2022
Int. Cl. C07K 16/28 (2006.01)
CPC C07K 16/2827 (2013.01) [C07K 16/2809 (2013.01); C07K 2317/24 (2013.01); C07K 2317/31 (2013.01); C07K 2317/76 (2013.01); C07K 2317/92 (2013.01)] 13 Claims
 
1. An antibody or an antigen-binding fragment thereof that specifically binds to an IgV domain of a B7-H3 protein;
wherein the antibody is monoclonal 8B12 or the antigen-binding fragment thereof is an antigen binding fragment of the monoclonal 8B12 antibody comprising:
(a) a VH region comprising: CDR1 consisting of the amino acid sequence set forth in SEQ ID NO: 1, CDR2 consisting of the amino acid sequence set forth in SEQ ID NO: 2, and CDR3 consisting of the amino acid sequence set forth in SEQ ID NO: 3; and
(b) a VL region comprising: CDR1 consisting of the amino acid sequence set forth in SEQ ID NO: 4, CDR2 consisting of the amino acid sequence set forth in SEQ ID NO: 5, and CDR3 consisting of the amino acid sequence set forth in SEQ ID NO: 6; or
wherein the antibody is monoclonal 12B4 or the antigen-binding fragment thereof is an antigen binding fragment of the monoclonal 12B4 antibody comprising:
(a) a VH region comprising: CDR1 consisting of the amino acid sequence set forth in SEQ ID NO: 7, CDR2 consisting of the amino acid sequence set forth in SEQ ID NO: 8, and CDR3 consisting of the amino acid sequence set forth in SEQ ID NO: 9; and
(b) a VL region comprising: CDR1 consisting of the amino acid sequence set forth in SEQ ID NO: 10, CDR2 consisting of the amino acid sequence set forth in SEQ ID NO: 11, and CDR3 consisting of the amino acid sequence set forth in SEQ ID NO: 12; or
wherein the antibody is monoclonal 24D12 or the antigen-binding fragment thereof is an antigen binding fragment of the monoclonal 24D12 antibody comprising:
(a) a VH region comprising: CDR1 consisting of the amino acid sequence set forth in SEQ ID NO: 7, CDR2 consisting of the amino acid sequence set forth in SEQ ID NO: 8, and CDR3 consisting of the amino acid sequence set forth in SEQ ID NO: 15; and
(b) a VL region comprising: CDR1 consisting of the amino acid sequence set forth in SEQ ID NO: 13, CDR2 consisting of the amino acid sequence set forth in SEQ ID NO: 14, and CDR3 consisting of the amino acid sequence set forth in SEQ ID NO: 16.