	US 12,252,529 B2
	Streptococcus pneumoniae antiserum without cross-reactivity and method for producing the same
Hun Kim, Seongnam-si (KR); Yoon Jae Lee, Seongnam-si (KR); Seung-Beom Paik, Seongnam-si (KR); and Jin-Hwan Shin, Seongnam-si (KR)
Assigned to SK BIOSCIENCE CO., LTD., Gyeonggi-Do (KR)
Appl. No. 17/429,089
Filed by SK BIOSCIENCE CO., LTD., Seongnam-si (KR)
PCT Filed Feb. 6, 2020, PCT No. PCT/KR2020/001707 § 371(c)(1), (2) Date Aug. 6, 2021, PCT Pub. No. WO2020/162689, PCT Pub. Date Aug. 13, 2020.
Claims priority of application No. 10-2019-0014539 (KR), filed on Feb. 7, 2019.
Prior Publication US 2022/0127339 A1, Apr. 28, 2022
Int. Cl. C07K 16/12 (2006.01); A61P 31/04 (2006.01); C07K 16/06 (2006.01); G01N 33/569 (2006.01)

CPC C07K 16/1275 (2013.01) [A61P 31/04 (2018.01); C07K 16/06 (2013.01); G01N 33/56944 (2013.01); C07K 2317/10 (2013.01); C07K 2317/33 (2013.01); C07K 2317/35 (2013.01)]

12 Claims

1. A method for preparing antiserum of Streptococcus pneumoniae, the method comprising the steps of:

(a) administering Streptococcus pneumoniae to a subject;

(b) collecting a serum from the subject;

(c) mixing the collected serum with Streptococcus pneumoniae of different serotype from the Streptococcus pneumoniae in the step (a) and confirming the presence or absence of cross-reactivity;

(d) removing an aggregated complex induced by cross-reactivity, in case where the presence of cross-reactivity is confirmed in the step (c); and

(e) obtaining the antiserum of which cross-reactivity is removed in the step (d).