	US 12,252,526 B2
	TCR-enriched clonotype, acquisition method and use thereof
Zhigang Xue, Hunan (CN); Jinfeng Xue, Hunan (CN); Ning Yi, Hunan (CN); Bo Lv, Hunan (CN); Chanyi Li, Hunan (CN); Lingbin Qi, Hunan (CN); and Weilin Li, Hunan (CN)
Assigned to HUNAN YUANPIN CELL TECHNOLOGY CO. LTD., Changsha (CN)
Filed by HUNAN YUANPIN CELL TECHNOLOGY CO. LTD., Hunan (CN)
Filed on Sep. 15, 2021, as Appl. No. 17/475,686.
Application 17/475,686 is a continuation in part of application No. PCT/CN2020/105806, filed on Jul. 30, 2020.
Claims priority of application No. 202010444541.9 (CN), filed on May 23, 2020; application No. 202010444542.3 (CN), filed on May 23, 2020; application No. 202010444546.1 (CN), filed on May 23, 2020; application No. 202010444548.0 (CN), filed on May 23, 2020; and application No. 202010445718.7 (CN), filed on May 23, 2020.
Prior Publication US 2021/0403529 A1, Dec. 30, 2021
Int. Cl. C07K 14/725 (2006.01); A61K 39/00 (2006.01)

CPC C07K 14/7051 (2013.01) [A61K 39/00 (2013.01)]

2 Claims

1. A method for acquiring TCR-enriched clonotypes, wherein the amino acid sequences of each pair of the TCR-enriched clonotypes are set forth in SEQ ID NO: 1 and SEQ ID NO: 2, SEQ ID NO: 3 and SEQ ID NO: 4, SEQ ID NO: 5 and SEQ ID NO: 6, SEQ ID NO: 7 and SEQ ID NO: 8, or SEQ ID NO: 9 and SEQ ID NO: 10, respectively;

and wherein the acquisition method comprises the following steps:

collecting peripheral blood mononuclear cell samples from a cured patient with viral infection;

conducting TCR/BCR V(D)J immune repertoire sequencing analysis on a collected sample to find a shared CDR3 variable region; and

detecting the distribution of cell clonotypes with high enrichment degree in the cured patient with viral infection to further obtain the TCR-enriched clonotypes.