US 12,252,521 B2
Binding domains directed against GPCR:G protein complexes and uses derived thereof
Jan Steyaert, Beersel (BE); Els Pardon, Wezemaal (BE); Toon Laeremans, Dworp (BE); Brian Kobilka, Palo Alto, CA (US); Soren G. F. Rasmussen, Frederiksberg (DK); Sebastien Granier, Menlo Park, CA (US); and Roger K Sunahara, Ann Arbor, MI (US)
Assigned to The Board of Trustees of the Leland Stanford Junior University, Palo Alto, CA (US); Vrije Universiteit Brussel, Brussels (BE); and The Regens of the University of Michigan, Ann Arbor, MI (US)
Filed by VIB VZW, Ghent (BE); Vrije Universiteit Brussel, Brussels (BE); The Board of Trustees of the Leland Stanford Junior University, Palo Alto, CA (US); and The Regents of the University of Michigan, Ann Arbor, MI (US)
Filed on Apr. 17, 2020, as Appl. No. 16/852,235.
Application 16/852,235 is a continuation of application No. 15/583,540, filed on May 1, 2017, granted, now 10,626,154.
Application 15/583,540 is a continuation of application No. 14/129,100, granted, now 9,695,227, issued on Jul. 4, 2017, previously published as PCT/EP2012/062036, filed on Jun. 21, 2012.
Claims priority of provisional application 61/571,159, filed on Jun. 21, 2011.
Claims priority of application No. 11181357 (EP), filed on Sep. 15, 2011.
Prior Publication US 2020/0239534 A1, Jul. 30, 2020
Int. Cl. C07K 14/47 (2006.01); C07K 14/435 (2006.01); C07K 14/705 (2006.01); C07K 16/28 (2006.01); G01N 33/74 (2006.01)
CPC C07K 14/4722 (2013.01) [C07K 14/435 (2013.01); C07K 14/705 (2013.01); C07K 14/70571 (2013.01); C07K 16/28 (2013.01); G01N 33/74 (2013.01); C07K 2317/22 (2013.01); C07K 2317/569 (2013.01); C12N 2799/026 (2013.01); G01N 2333/726 (2013.01); G01N 2500/02 (2013.01)] 11 Claims
 
1. A method for identifying a test compound that binds to a GPCR/G-protein complex, the method comprising:
determining that a protein binding domain binds to the G-protein in the GPCR/G-protein complex and stabilizes the GPCR/G-protein complex, wherein the protein binding domain is a nanobody;
forming a complex of the GPCR, the G-protein, and the protein binding domain;
contacting the complex with the test compound; and
determining binding between the test compound and the complex.