US 12,252,517 B2
Hook fusion protein for regulating the cellular trafficking of a target protein
Franck Perez, Paris (FR); Zelia Gouveia, L'hay les Roses (FR); and Gaelle Boncompain, Chatillon (FR)
Assigned to INSTITUT CURIE, Paris (FR); and CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE—CNRS, Paris (FR)
Filed by INSTITUT CURIE, Paris (FR); and CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS), Paris (FR)
Filed on Sep. 11, 2023, as Appl. No. 18/464,507.
Application 18/464,507 is a continuation of application No. 16/757,410, abandoned, previously published as PCT/EP2018/078930, filed on Oct. 22, 2018.
Claims priority of application No. 17306453 (EP), filed on Oct. 20, 2017.
Prior Publication US 2024/0018197 A1, Jan. 18, 2024
Int. Cl. C07K 14/725 (2006.01); A61K 39/00 (2006.01); C07K 14/36 (2006.01); C07K 14/705 (2006.01); C07K 14/74 (2006.01); C12N 15/63 (2006.01)
CPC C07K 14/36 (2013.01) [A61K 39/0011 (2013.01); C07K 14/7051 (2013.01); C07K 14/70539 (2013.01); C07K 14/7056 (2013.01); C12N 15/63 (2013.01); A61K 2039/5156 (2013.01); A61K 2039/5158 (2013.01); C07K 2319/04 (2013.01); C07K 2319/10 (2013.01)] 9 Claims
 
1. A hook fusion protein comprising:
a hook domain which is a streptavidin; and
at least one cytoplasmic carboxy terminal endoplasmic reticulum (ER) retention signal or at least one cytoplasmic amino terminal endoplasmic reticulum (ER) retention signal, or a combination thereof;
wherein the hook fusion protein is a soluble protein that localizes in the cytoplasm, and wherein the hook fusion protein does not comprise a transmembrane domain.