US 12,252,513 B2
Thermostable phycobiliproteins produced from recombinant arthrospira
James Roberts, Seattle, WA (US); Ryo Takeuchi, Seattle, WA (US); Nhi Khuong, Kenmore, WA (US); Barry L Stoddard, Bellevue, WA (US); and Rolf Kuestner, Seattle, WA (US)
Assigned to Lumen Bioscience, Inc., Seattle, WA (US)
Appl. No. 17/260,693
Filed by LUMEN BIOSCIENCE, INC., Seattle, WA (US)
PCT Filed Jul. 16, 2019, PCT No. PCT/US2019/042072
§ 371(c)(1), (2) Date Jan. 15, 2021,
PCT Pub. No. WO2020/018586, PCT Pub. Date Jan. 23, 2020.
Claims priority of provisional application 62/698,712, filed on Jul. 16, 2018.
Prior Publication US 2022/0135627 A1, May 5, 2022
Int. Cl. C07K 14/195 (2006.01); C12N 1/20 (2006.01)
CPC C07K 14/195 (2013.01) [C12N 1/20 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A thermostable phycobiliprotein modified for greater thermostability by the formation of covalent disulfide bonds, wherein the covalent disulfide bonds are formed by replacing one or more alanine, aspartic acid, or serine residues with one or more cysteine residues in the modified phycobiliprotein, wherein the covalent disulfide bonds are between residues that are within: alpha-helices α2 and α7, of an phycocyanin alpha subunit; or an alpha-helix α1 of the phycocyanin alpha subunit and an N-terminal region of a phycocyanin beta subunit upstream of the alpha-helix α1 the helices determined by alignment with a sequence selected from: SEQ ID NO: 35-36 or 41-42 of the phycocyanin alpha subunit or a sequence selected from SEQ ID NO: 37-40 of the phycocyanin beta subunit.