US 12,252,510 B2
Method for purifying antibody or antibody-like molecule
Fuminori Konoike, Takasago (JP); and Kazunobu Minakuchi, Takasago (JP)
Assigned to KANEKA CORPORATION, Osaka (JP)
Appl. No. 17/268,637
Filed by KANEKA CORPORATION, Osaka (JP)
PCT Filed Aug. 23, 2019, PCT No. PCT/JP2019/033086
§ 371(c)(1), (2) Date Feb. 16, 2021,
PCT Pub. No. WO2020/045290, PCT Pub. Date Mar. 5, 2020.
Claims priority of application No. 2018-163228 (JP), filed on Aug. 31, 2018.
Prior Publication US 2021/0198309 A1, Jul. 1, 2021
Int. Cl. C07K 1/34 (2006.01); C07K 1/02 (2006.01); C07K 16/00 (2006.01)
CPC C07K 1/34 (2013.01) [C07K 1/02 (2013.01); C07K 16/00 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method for purifying an antibody or a protein, the method comprising the step of:
adding a pre-formed water-insoluble inorganic compound to an aqueous solution or a suspension comprising the antibody or the protein and an impurity or filling a column with a pre-formed water-insoluble inorganic compound and passing an aqueous solution or a suspension comprising the antibody or the protein through the column to adsorb the impurity onto said pre-formed water-insoluble inorganic compound and obtain a liquid part comprising the antibody or the protein,
wherein said pre-formed water-insoluble inorganic compound comprises at least one member selected from the group consisting of magnesium carbonate, magnesium oxide, magnesium phosphate, and aluminum oxide, and
the antibody or the protein is at least one member selected from the group consisting of polyclonal antibody, monoclonal antibody, human antibody, humanized antibody, chimeric antibody, single-chain antibody, heavy chain antibody, multivalent antibody, Fab, F(ab′), F(ab′)2, Fc, Fc fusion protein, bispecific antibody, heavy chain, light chain, single-chain Fv, sc(Fv)2, disulfide bonded Fv, Diabody or micro-antibody.