US 12,251,700 B2
Thermal cycler
Bernd Gröhbühl, Ettlingen (DE); and Martin Trump, Pforzheim (DE)
Assigned to STRATEC SE, Birkenfeld (DE)
Filed by STRATEC SE, Birkenfeld (DE)
Filed on Oct. 25, 2018, as Appl. No. 16/170,319.
Claims priority of application No. 17198361 (EP), filed on Oct. 25, 2017; and application No. 100593 (LU), filed on Dec. 22, 2017.
Prior Publication US 2019/0118183 A1, Apr. 25, 2019
Int. Cl. B01L 7/00 (2006.01); C12Q 1/686 (2018.01)
CPC B01L 7/52 (2013.01) [C12Q 1/686 (2013.01); B01L 2200/026 (2013.01); B01L 2200/0684 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/18 (2013.01); B01L 2300/1827 (2013.01); B01L 2300/185 (2013.01); B01L 2300/1894 (2013.01); B01L 2400/0622 (2013.01); B01L 2400/0638 (2013.01); B01L 2400/0644 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A system for thermal cycling, comprising:
at least one separate vessel holder for taking up a single reaction vessel and providing a contact surface for the single reaction vessel, wherein each of the at least one separate single vessel holder comprises:
an internal individual vertical meandering fluidic path in a descending flow path around the reaction vessel, configured for supplying a cooling liquid or pressurized air on one side of said at least one separate vessel holder from its upper to its lower side and on its opposite side from its lower to its upper side; and
at least one individual electrical heater attached on opposite sides next to the at least one separate vessel holder for individually adjusting the temperature of the single reaction vessel;
a multilayer liquid port with at least one sealing contact surface for the at least one separate vessel holder and inlet and outlet openings for the attachment of the at least one separate vessel holder configured for providing the cooling liquid or the pressurized air to the at least one separate vessel holder;
a 3-port/2-way control valve inside the multilayer liquid port, such control valve connected to (i) a first membrane valve connected to liquid and (ii) a second membrane valve connected to pressurized air, wherein the first and second membrane valves are 2-port/2-way valves and are alternatingly switched on and off by the control valve and the default position of the control valve is pressurized air; and
at least one control unit for connecting with the at least one electrical heater.