| CPC B01L 3/502 (2013.01) [B01L 2300/044 (2013.01); B01L 2300/0672 (2013.01)] | 20 Claims |

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1. A hand-held liquid handling device for diluting a liquid sample, the device comprising:
two chambers connected together and arranged to contain liquid, wherein the two chambers include a housing for a liquid transfer mechanism; and
a first liquid transfer mechanism arranged to transfer a predetermined volume of liquid from one chamber to an adjacent chamber, the first liquid transfer mechanism including:
a rotatable container having a predetermined volume, wherein the rotatable container is insertable into the housing;
a handle, coupled to one end of the rotatable container, extending outside of the device for rotating the rotatable container;
a plurality of resiliently deformable fingers disposed around another end of the rotatable container to hold the rotatable container within the housing; and
a locking mechanism for preventing movement of the rotatable container in the housing after the predetermined volume of liquid has been transferred from one chamber to the adjacent chamber.
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