US 12,251,681 B2
Method of manufacturing agar or agarose beads
Susanna Lindberg, Uppsala (SE); Jonas Gustafsson, Uppsala (SE); Linn Carlsson, Uppsala (SE); Anders Hagvall, Uppsala (SE); Erik Ringberg, Uppsala (SE); and David Jansson, Uppsala (SE)
Assigned to Cytiva BioProcess R&D AB, Uppsala (SE)
Appl. No. 17/605,502
Filed by Cytiva Bioprocess R&D AB, Uppsala (SE)
PCT Filed Apr. 28, 2020, PCT No. PCT/EP2020/061790
§ 371(c)(1), (2) Date Oct. 21, 2021,
PCT Pub. No. WO2020/221762, PCT Pub. Date Nov. 5, 2020.
Claims priority of application No. 1905919 (GB), filed on Apr. 29, 2019.
Prior Publication US 2022/0219141 A1, Jul. 14, 2022
Int. Cl. B01J 20/29 (2006.01); B01J 20/24 (2006.01); B01J 20/28 (2006.01); B01J 20/291 (2006.01); B01J 20/30 (2006.01)
CPC B01J 20/291 (2013.01) [B01J 20/24 (2013.01); B01J 20/28019 (2013.01); B01J 20/28047 (2013.01); B01J 20/3007 (2013.01); B01J 20/3085 (2013.01); B01J 2220/52 (2013.01)] 28 Claims
OG exemplary drawing
 
1. A method for manufacturing agar or agarose beads, comprising the steps of:
a) providing a water phase comprising an aqueous solution of agar or agarose at a temperature of 40-100° C.;
b) providing an oil phase comprising a water-immiscible solvent and an emulsifier at a temperature of 40-100° C.;
c) emulsifying said water phase in said oil phase to form a water-in-oil emulsion;
d) cooling said water-in-oil emulsion to a temperature below a gelation temperature of said agar or agarose to form a dispersion of solidified agar or agarose beads; and
e) recovering agar or agarose beads from said dispersion,
wherein said emulsifier comprises a phosphate ester of an alkoxylated fatty alcohol.