US 12,251,439 B2
Inactivating bacteria with electric pulses and antibiotics
Allen Lawrence Garner, West Lafayette, IN (US); Ram Anand Vadlamani, West Lafayette, IN (US); and David Alan Detwiler, Columbia City, IN (US)
Assigned to Purdue Research Foundation, West Lafayette, IN (US); and Nanovis, LLC, Columbia City, IN (US)
Filed by Purdue Research Foundation, West Lafayette, IN (US); and Nanovis, LLC, Columbia City, IN (US)
Filed on Oct. 19, 2023, as Appl. No. 18/490,238.
Application 18/490,238 is a continuation of application No. 17/461,143, filed on Aug. 30, 2021, granted, now 11,844,835.
Application 17/461,143 is a continuation of application No. 16/991,284, filed on Aug. 12, 2020, granted, now 11,123,428, issued on Sep. 21, 2021.
Application 16/991,284 is a continuation of application No. PCT/US2019/017761, filed on Feb. 13, 2019.
Claims priority of provisional application 62/630,219, filed on Feb. 13, 2018.
Prior Publication US 2024/0066128 A1, Feb. 29, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 41/00 (2020.01); A61K 31/341 (2006.01); A61K 31/496 (2006.01); A61K 31/5377 (2006.01); A61K 31/575 (2006.01); A61K 31/702 (2006.01); A61K 31/7048 (2006.01); A61K 38/14 (2006.01); A61N 1/32 (2006.01); C12N 13/00 (2006.01)
CPC A61K 41/0023 (2013.01) [A61K 31/341 (2013.01); A61K 31/496 (2013.01); A61K 31/5377 (2013.01); A61K 31/575 (2013.01); A61K 31/702 (2013.01); A61K 31/7048 (2013.01); A61K 38/14 (2013.01); A61N 1/32 (2013.01); C12N 13/00 (2013.01)] 20 Claims
 
1. A method of increasing the antimicrobial effect of an antibiotic compound in viable microbes comprising:
applying an amount of an antibiotic compound to the viable microbes; and
applying pulses of electricity to the viable microbes having a duration of between about 50 nanoseconds and about 900 nanoseconds.