US 12,251,433 B2
Engineering gut commensal bacteria to express heterologous proteins in their outer membrane vesicles (OMVS) for delivery to the GI-tract
Regis Gabriel Stentz, Norwich (GB); and Simon Richard Carding, Norwich (GB)
Assigned to QUADRAM INSTITUTE BIOSCIENCE, Norwich (GB); and UEA ENTERPRISES LIMITED, Norwich (GB)
Filed by QUADRAM INSTITUTE BIOSCIENCE, Norwich (GB); and UEA ENTERPRISES LIMITED, Norwich (GB)
Filed on May 10, 2021, as Appl. No. 17/316,219.
Application 17/316,219 is a division of application No. 16/086,906, granted, now 11,000,581, previously published as PCT/GB2017/051199, filed on Apr. 28, 2017.
Claims priority of application No. 1607510 (GB), filed on Apr. 29, 2016.
Prior Publication US 2021/0268094 A1, Sep. 2, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 39/39 (2006.01); A61K 35/74 (2015.01); A61K 39/02 (2006.01); A61K 39/112 (2006.01); A61P 31/04 (2006.01); C12N 1/20 (2006.01); C12N 9/24 (2006.01); C12N 15/52 (2006.01); C12N 15/74 (2006.01); C12P 21/02 (2006.01); A61K 38/00 (2006.01); A61K 39/00 (2006.01)
CPC A61K 39/0216 (2013.01) [A61K 35/74 (2013.01); A61K 39/0275 (2013.01); A61P 31/04 (2018.01); C12N 1/20 (2013.01); C12N 9/2488 (2013.01); C12N 15/52 (2013.01); C12N 15/74 (2013.01); C12P 21/02 (2013.01); A61K 38/00 (2013.01); A61K 2039/541 (2013.01); A61K 2039/6068 (2013.01); C07K 2319/02 (2013.01)] 13 Claims
 
1. A recombinant gram negative commensal gut bacterium from the Bacteroides genus comprising an expression system for expression of a heterologous peptide or protein, wherein said heterologous peptide or protein is contained in an outer membrane vesicle (OMV), characterised in that the heterologous peptide or protein is fused to the N-terminal signal peptide of the OMV-secreted protein Bt OmpA.