| CPC A61B 5/0071 (2013.01) [A61B 5/0059 (2013.01); A61B 5/01 (2013.01); A61B 5/445 (2013.01); A61B 5/72 (2013.01); A61B 5/742 (2013.01); G01N 21/6456 (2013.01); G01N 21/6486 (2013.01); A61B 5/0042 (2013.01); A61B 5/4519 (2013.01); A61B 10/00 (2013.01); G01N 21/6408 (2013.01); G01N 2021/6421 (2013.01); G01N 2021/6471 (2013.01); G01N 2201/0221 (2013.01); Y02A 90/10 (2018.01)] | 21 Claims |

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1. A portable, handheld system for acquiring data and outputting a representation regarding a tissue, comprising:
a housing configured to removably receive at least a portion of a wireless communication device;
at least one excitation light source configured to illuminate a target tissue with excitation light, the at least one excitation light source being coupled to the housing and the target tissue being a tissue including a wound;
an optical sensor configured to detect signals responsive to illumination of the target tissue, each signal indicative of at least one of endogenous fluorescence, exogenous fluorescence, absorbance, and reflectance in, from or emitted by the target tissue;
a thermal sensor configured to detect thermal information regarding the target tissue;
a processor configured to receive the detected signals and the detected thermal information and to output the representation regarding the target tissue, the output representation including wound size and at least one temperature associated with the wound; and
a display for displaying the representation regarding the target tissue output by the processor, wherein the processor is further configured to:
identify, based on the detected signals and/or the output representation, one or more of a presence, a location, a distribution, and an extent of one or more of collagen, elastin, connective tissue, blood, bone, exudate, stromal tissue, granulation tissue, and other tissue, cells, molecules, and fluids indicative of infection and/or healing present in and/or on the target tissue, and
calculate red and green fluorescence intensities, and use the calculated red and green fluorescence intensities:
to produce image maps of fluorescence intensities of the target tissue, displayed in color or pseudo color; and
identify the presence and biodistribution of bacteria within the wound.
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