| CPC A61B 5/0066 (2013.01) [A61B 5/0084 (2013.01)] | 18 Claims |

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1. An apparatus, comprising:
an optical probe having a first light to illuminate a sample;
a detector for detecting a fluorescence of reflection of the first light for illuminating the sample;
a sheath configured to allow the optical probe to travel longitudinally through the sheath to perform a pullback,
wherein the sheath has a fluorescent coating of known emitted fluorescence or known range of emitted fluorescence on at least a portion of the sheath surface for detection by the detector, and
a processor configured to
receive an emitted fluorescence measurement value from the fluorescent coating
determine a slope of a linear response for the emitted fluorescence measurement value, and
use the slope of a linear response to calibrate a fluorescence linear response.
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