CPC C12N 15/907 (2013.01) [C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12N 15/902 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01)] | 28 Claims |
1. A nucleic acid molecule comprising a polynucleotide encoding an RNA-guided nuclease (RGN) polypeptide, wherein said polynucleotide comprises a nucleotide sequence encoding an RGN polypeptide comprising an amino acid sequence:
(i) having at least 95% sequence identity to any one of SEQ ID NOs: 11, 27, 45, or 54;
(ii) having at least 98% sequence identity to SEQ ID NOs: 1 or 19; or
(iii) set forth as SEQ ID NO: 36;
wherein said polynucleotide encoding an RGN polypeptide is operably linked to a promoter heterologous to said polynucleotide.
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