CPC C12N 15/86 (2013.01) [C12Q 1/025 (2013.01); C12Q 1/6897 (2013.01); C12Q 1/70 (2013.01); C12N 2750/14143 (2013.01); C12N 2750/14152 (2013.01); C12N 2830/002 (2013.01)] | 17 Claims |
1. A circular nucleic acid comprising in 5′ to 3′ direction
(i) at least one adeno-associated virus (AAV) inverted terminal repeat (ITR) sequence;
(ii) at least one AAV promoter which is capable of being activated by at least one helper polypeptide or helper polynucleotide originating or derived from a virus selected from Adenoviridae and Herpesviridae;
(iii) at least one AAV rep gene coding sequence under the control of said promoter of (ii);
(iv) an AAV promoter which is capable of being activated by said at least one helper polypeptide or helper polynucleotide originating from a virus selected from Adenoviridae and Herpesviridae;
(v) a transgenic coding sequence under the control of said promoter of (iv); and
(vi) an adeno-associated virus (AAV) inverted terminal repeat (ITR) sequence;
wherein said circular nucleic acid does not comprise an AAV cap gene.
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