US 11,926,833 B2
Compositions and methods for enhancing biomass productivity in plants
Madeline Hall, Hayward, CA (US); Li-Wei Chiu, Hayward, CA (US); Rebecca Dewhirst, Hayward, CA (US); Jacob Hoyle, Hayward, CA (US); Patrick Mellor, Hayward, CA (US); Karli Rasmussen, Hayward, CA (US); and Yumin Tao, Hayward, CA (US)
Assigned to LIVING CARBON PBC, Haywood, CA (US)
Filed by Living Carbon PBC, Hayward, CA (US)
Filed on Jan. 12, 2023, as Appl. No. 18/153,863.
Claims priority of provisional application 63/302,895, filed on Jan. 25, 2022.
Prior Publication US 2023/0235336 A1, Jul. 27, 2023
Int. Cl. C12N 15/52 (2006.01); C12N 15/113 (2010.01)
CPC C12N 15/52 (2013.01) [C12N 15/113 (2013.01); C12N 2310/11 (2013.01)] 26 Claims
 
1. A transgenic tree plant cell comprising:
endogenous glycolate transporter Plgg1 and
one or more heterologous polynucleotides comprising:
a first promoter operably linked to a polynucleotide sequence encoding a glycolate dehydrogenase;
a second promoter operably linked to a polynucleotide sequence encoding a malate synthase; and
an RNAi polynucleotide targeting the endogenous glycolate transporter Plgg1, wherein the RNAi polynucleotide comprises a sense strand comprising at least 21 contiguous nucleotides of the endogenous glycolate transporter Plgg1 and an antisense strand that is fully complementary to the at least 21 contiguous nucleotides of the sense strand, wherein the sense strand and the antisense strand form a dsRNA, wherein expression of the endogenous glycolate transporter Plgg1 in the transgenic tree plant cell is about 20% to 80% of expression of endogenous glycolate transporter Plgg1 in a tree plant cell that is not transformed with the RNAi polynucleotide targeting the endogenous glycolate transporter Plgg1, and wherein the transgenic tree plant cell is from a tree selected from Populus, Pinus taeda, and Picea abies.