CPC C07K 16/2809 (2013.01) [A61P 35/00 (2018.01); C07K 16/28 (2013.01); C07K 16/3053 (2013.01); A61K 2039/505 (2013.01); C07K 2317/31 (2013.01); C07K 2317/33 (2013.01); C07K 2317/56 (2013.01); C07K 2317/565 (2013.01); C07K 2317/622 (2013.01); C07K 2317/73 (2013.01); C07K 2317/732 (2013.01); C07K 2317/92 (2013.01); C07K 2317/94 (2013.01)] | 22 Claims |
1. A method for treating or ameliorating a tumor or cancer, the method comprising administering to a subject in need thereof a bispecific single chain antibody construct comprising:
a first human binding domain which binds to human CDH3 on the surface of a target cell; and
a second binding domain which binds to human CD3 on the surface of a T cell,
wherein the first binding domain comprises a VH region and a VL region selected from the group consisting of:
a) a VH region comprising a CDR-H1 comprising the amino acid sequence of SEQ ID NO: 169, a CDR-H2 comprising the amino acid sequence of SEQ ID NO: 170, and a CDR-H3 comprising the amino acid sequence of SEQ ID NO: 171, and a VL region comprising a CDR-L1 comprising the amino acid sequence of SEQ ID NO: 172, a CDR-L2 comprising the amino acid sequence of SEQ ID NO: 173, and a CDR-L3 comprising the amino acid sequence of SEQ ID NO: 174;
b) a VH region comprising a CDR-H1 comprising the amino acid sequence of SEQ ID NO: 279, a CDR-H2 comprising the amino acid sequence of SEQ ID NO: 280, and a CDR-H3 comprising the amino acid sequence of SEQ ID NO: 281, and a VL region comprising a CDR-L1 comprising the amino acid sequence of SEQ ID NO: 282, a CDR-L2 comprising the amino acid sequence of SEQ ID NO: 283, and a CDR-L3 comprising the amino acid sequence of SEQ ID NO: 284;
c) a VH region comprising a CDR-H1 comprising the amino acid sequence of SEQ ID NO: 289, a CDR-H2 comprising the amino acid sequence of SEQ ID NO: 290, and a CDR-H3 comprising the amino acid sequence of SEQ ID NO: 291, and a VL region comprising a CDR-L1 comprising the amino acid sequence of SEQ ID NO: 292, a CDR-L2 comprising the amino acid sequence of SEQ ID NO: 293, and a CDR-L3 comprising the amino acid sequence of SEQ ID NO: 294;
d) a VH region comprising a CDR-H1 comprising the amino acid sequence of SEQ ID NO: 299, a CDR-H2 comprising the amino acid sequence of SEQ ID NO: 300, and a CDR-H3 comprising the amino acid sequence of SEQ ID NO: 301, and a VL region comprising a CDR-L1 comprising the amino acid sequence of SEQ ID NO: 302, a CDR-L2 comprising the amino acid sequence of SEQ ID NO: 303, and a CDR-L3 comprising the amino acid sequence of SEQ ID NO: 304; and
e) a VH region comprising a CDR-H1 comprising the amino acid sequence of SEQ ID NO: 309, a CDR-H2 comprising the amino acid sequence of SEQ ID NO: 310, and a CDR-H3 comprising the amino acid sequence of SEQ ID NO: 311, and a VL region comprising a CDR-L1 comprising the amino acid sequence of SEQ ID NO: 312, a CDR-L2 comprising the amino acid sequence of SEQ ID NO: 313, and a CDR-L3 comprising the amino acid sequence of SEQ ID NO: 314.
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