CPC A61K 39/39533 (2013.01) [A61K 39/3955 (2013.01); C07K 16/22 (2013.01); G01N 33/53 (2013.01); G01N 33/74 (2013.01); A61K 2039/505 (2013.01); C07K 14/475 (2013.01); C07K 2317/21 (2013.01); C07K 2317/30 (2013.01); C07K 2317/33 (2013.01); C07K 2317/622 (2013.01); C07K 2317/76 (2013.01); C07K 2317/92 (2013.01); C07K 2317/94 (2013.01); G01N 2800/10 (2013.01)] | 9 Claims |
1. A host cell comprising a polynucleotide encoding an antibody or antigen-binding fragment thereof that specifically binds to pro/latent myostatin, binds to pro/latent myostatin with a decreased affinity at an acidic pH as compared to a neutral or physiological pH, and inhibits myostatin signaling after binding, wherein the host cell is prepared by the steps of:
(i) screening for an antibody or antigen-binding fragment that specifically binds to pro/latent myostatin as compared to mature myostatin and/or another member of the transforming growth factor beta family;
(ii) screening the antibody or antigen-binding fragment thereof for inhibition of myostatin signaling;
(iii) assaying the antibody or antigen-binding fragment for binding to pro/latent myostatin wherein the antibody or fragment binds to pro/latent myostatin with a decreased affinity at an acidic pH as compared to a neutral or physiological pH;
(iv) isolating a polynucleotide encoding the antibody or antigen-binding fragment; and
(v) incorporating the polynucleotide in the host cell.
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