	US 11,925,668 B2
	Postbiotic extract and preparation process and use thereof
Meei-Yn Lin, Taichung (TW); Hung-Pin Chiu, Taichung (TW); and Yi-Heng Chiu, Taichung (TW)
Assigned to CHAMBIO CO., LTD., Taichung (TW)
Filed by CHAMBIO CO., LTD., Taichung (TW)
Filed on Nov. 2, 2022, as Appl. No. 18/051,983.
Application 18/051,983 is a division of application No. 17/037,090, filed on Sep. 29, 2020, granted, now 11,541,085.
Prior Publication US 2023/0293606 A1, Sep. 21, 2023
Int. Cl. A61K 35/747 (2015.01); A01N 63/20 (2020.01); A23L 33/135 (2016.01); A61K 35/00 (2006.01); A61K 35/741 (2015.01); A61K 35/745 (2015.01); A61K 39/085 (2006.01); A61K 47/36 (2006.01); A61K 47/42 (2017.01); A61K 35/744 (2015.01)

CPC A61K 35/747 (2013.01) [A01N 63/20 (2020.01); A23L 33/135 (2016.08); A61K 35/741 (2013.01); A61K 35/745 (2013.01); A61K 39/085 (2013.01); A61K 47/36 (2013.01); A61K 47/42 (2013.01); A23V 2002/00 (2013.01); A23V 2400/113 (2023.08); A23V 2400/127 (2023.08); A23V 2400/517 (2023.08); A23V 2400/531 (2023.08); A23V 2400/533 (2023.08); A61K 2035/115 (2013.01); A61K 35/744 (2013.01)]

1 Claim

1. A method of improving gut health, comprising administering to a subject in need thereof a postbiotic extract, wherein the postbiotic extract is prepared by a process including the steps of:

a) providing a whey protein having an isoelectric point of 4.4 and a dextrin having an isoelectric point of 5.2;

b) admixing the whey protein and a probiotic bacterium with water having a pH value of 7.5, so as to form a mixture,

wherein the probiotic bacterium is selected from the group consisting of Lactobacillus plantarum CB102, Lactobacillus acidophilus JCM1132, Lactobacillus casei JCM1134, Bifidobacterium bifidum JCM1255, Bifidobacterium lactis JCM10602, Bifidobacterium longum CB108, and combinations thereof;

c) adding the dextrin into the mixture, followed by adjusting the pH value of the dextrin-added mixture to between 4.4 and 5.2, so that a precipitate is formed; and

d) subjecting the precipitate to an acid hydrolysis treatment with lactic acid to isolate the cell wall of the probiotic bacterium, so as to obtain the postbiotic extract.