CPC A01K 67/0278 (2013.01) [A01K 67/027 (2013.01); A01K 67/0275 (2013.01); C07K 16/00 (2013.01); C07K 16/462 (2013.01); C12N 5/10 (2013.01); C12N 15/8509 (2013.01); A01K 2207/15 (2013.01); A01K 2217/052 (2013.01); A01K 2217/075 (2013.01); A01K 2217/15 (2013.01); A01K 2217/206 (2013.01); A01K 2227/105 (2013.01); A01K 2267/01 (2013.01); C07K 14/47 (2013.01); C07K 16/005 (2013.01); C07K 16/10 (2013.01); C07K 16/1282 (2013.01); C07K 16/22 (2013.01); C07K 16/248 (2013.01); C07K 16/2863 (2013.01); C07K 16/32 (2013.01); C07K 2317/10 (2013.01); C07K 2317/14 (2013.01); C07K 2317/21 (2013.01); C07K 2317/24 (2013.01); C07K 2317/31 (2013.01); C07K 2317/34 (2013.01); C07K 2317/51 (2013.01); C07K 2317/515 (2013.01); C07K 2317/52 (2013.01); C07K 2317/55 (2013.01); C07K 2317/56 (2013.01); C07K 2317/622 (2013.01); C07K 2317/64 (2013.01); C07K 2317/76 (2013.01); C07K 2317/94 (2013.01); C07K 2319/00 (2013.01); C12P 21/00 (2013.01); G01N 33/56966 (2013.01)] | 6 Claims |
1. A transgenic murine animal that has been immunized to generate an immune response against an antigen, wherein the genome of said animal comprises a transgene comprising a single human immunoglobulin light chain V gene segment fused to a single human immunoglobulin light chain J gene segment,
such that said fused V/J gene segments encode a rearranged immunoglobulin light chain variable region, wherein the transgene lacks a regulatory element that contributes to somatic hypermutation of the light chain variable region; and
said transgenic animal comprising a population of B cells that secrete a repertoire of antibodies that bind said antigen, said repertoire of antibodies comprising the immunoglobulin light chain variable region encoded by said transgene and a light chain constant region, paired with a diversity of immunoglobulin heavy chains resulting from somatic hypermutation in response to said antigen.
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