US 12,249,085 B2
Sample handling apparatus and image registration methods
Augusto Manuel Tentori, Dublin, CA (US); Hanyoup Kim, Foster City, CA (US); Siyuan Xing, Newark, CA (US); Felice Alessio Bava, Pleasanton, CA (US); Rajiv Bharadwaj, Pleasanton, CA (US); Cedric Uytingco, Pleasanton, CA (US); and Erica Graziosa, Pleasanton, CA (US)
Assigned to 10x Genomics, Inc., Pleasanton, CA (US)
Filed by 10x Genomics, Inc., Pleasanton, CA (US)
Filed on Mar. 17, 2023, as Appl. No. 18/185,599.
Application 18/185,599 is a continuation of application No. PCT/US2021/050929, filed on Sep. 17, 2021.
Claims priority of provisional application 63/155,173, filed on Mar. 1, 2021.
Claims priority of provisional application 63/080,547, filed on Sep. 18, 2020.
Prior Publication US 2023/0267625 A1, Aug. 24, 2023
Int. Cl. G06V 10/24 (2022.01); G06T 7/33 (2017.01); G06V 10/25 (2022.01); G01N 1/30 (2006.01)
CPC G06T 7/33 (2017.01) [G06V 10/25 (2022.01); G01N 1/30 (2013.01); G06T 2207/10056 (2013.01); G06T 2207/10064 (2013.01); G06T 2207/20132 (2013.01); G06T 2207/30024 (2013.01); G06T 2207/30072 (2013.01); G06T 2207/30204 (2013.01)] 27 Claims
OG exemplary drawing
 
1. A method for aligning a sample to an array, the method comprising:
receiving, by a data processor, sample image data comprising a sample image of the sample on a first substrate, the sample image having a first resolution;
receiving, by the data processor, array image data comprising an array image having a second resolution lower than the first resolution, wherein the array image comprises an overlay of the array and an array fiducial on a second substrate with the sample on the first substrate, the array fiducial comprising a plurality of array fiducial elements separated from the array and forming a fiducial frame surrounding a perimeter of the array;
registering, by the data processor, the sample image to the array image by aligning the sample image and the array image using the array fiducial;
generating, by the data processor, an aligned image based on the registering, the aligned image comprising an overlay of the sample image with the array image; and
providing, by the data processor, the aligned image.