US 12,248,136 B2
Apparatus and method for simultaneous imaging and execution of contact-free directed hydrodynamic flow
Moritz Kreysing, Dresden (DE); Anatol Fritsch, Dresden (DE); and Matthaeus Mittasch, Dresden (DE)
Assigned to Max-Planck-Gesellschaft zur Foerderung der Wissenschaften E.V., Munich (DE)
Filed by Max-Planck-Gesellschaft zur Förderung der Wissenschaften E.V., Munich (DE)
Filed on Oct. 30, 2023, as Appl. No. 18/497,032.
Application 18/497,032 is a continuation of application No. 16/766,456, granted, now 11,803,046, previously published as PCT/EP2018/082441, filed on Nov. 23, 2018.
Claims priority of application No. 17203545 (EP), filed on Nov. 24, 2017.
Prior Publication US 2024/0142761 A1, May 2, 2024
Int. Cl. G02B 21/36 (2006.01); B01L 3/00 (2006.01); G01N 21/64 (2006.01); G02B 21/00 (2006.01); G02B 21/02 (2006.01); G02B 21/08 (2006.01); G02B 21/28 (2006.01); G02B 21/30 (2006.01); G01N 15/01 (2024.01)
CPC G02B 21/36 (2013.01) [B01L 3/50273 (2013.01); G02B 21/0032 (2013.01); G02B 21/02 (2013.01); G02B 21/08 (2013.01); G02B 21/28 (2013.01); G02B 21/30 (2013.01); B01L 3/5027 (2013.01); B01L 2400/0463 (2013.01); G01N 15/01 (2024.01); G01N 21/6458 (2013.01)] 20 Claims
OG exemplary drawing
 
1. Method for simultaneous imaging and executing contact-free directed hydrodynamic flow in a specimen comprising:
fluorescence imaging the specimen via an objective of a microscope using visible light provided by a light source,
dynamically heating the interior and/or a surface of the specimen via a light beam from an infrared laser source having a wavelength in the range of 1000 nm and 0.1 mm,
inducing a hydrodynamic flow in the specimen through changing heat patterns within the specimen by manipulating a specified location of the specimen which is to be heated by variably guiding the light beam to specified locations of the specimen,
coupling the light beam of the infrared laser source into an optical path of the microscope downstream of a manipulator for manipulating the specified location of the specimen which is to be heated,
guiding the light beam of the infrared laser source to the specimen through the objective of the microscope, and
inducing an oscillatory flow with a given frequency within the specimen by scanning the light beam multiple times unidirectionally and then reversing the unidirectional scan.