US 12,248,133 B2
Methods and apparatus for in vivo high resolution multiphoton microscopy
Jianan Qu, Hong Kong (CN); and Zhongya Qin, Hong Kong (CN)
Assigned to The Hong Kong University of Science and Technology, Hong Kong (CN)
Filed by The Hong Kong University of Science and Technology, Hong Kong (CN)
Filed on Nov. 17, 2021, as Appl. No. 17/529,219.
Claims priority of provisional application 63/140,261, filed on Jan. 22, 2021.
Prior Publication US 2022/0236547 A1, Jul. 28, 2022
Int. Cl. G02B 21/00 (2006.01); G02B 27/00 (2006.01)
CPC G02B 21/006 (2013.01) [G02B 21/0048 (2013.01); G02B 21/008 (2013.01); G02B 27/0068 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method for identifying and correcting optical aberrations within a sample under optical microscopy, comprising:
providing a plurality of optical beams including at least a first optical beam and a second optical beam;
modulating at least one of the optical beams at one or more frequencies, the intensity of one of the optical beams being higher than the other;
the first optical beam and the second optical beam being at least partially superimposed in time to provide a combined optical beam;
focusing the combined optical beam into an imaging sample, followed by detecting a first signal excited by the combined optical beam in the sample;
demodulating the first signal by at least one lock-in amplifier to obtain a second signal comprising performing a plurality of measurements of the spatial positions of the first optical beam with respect to the second optical beam; and
obtaining an electric-field point spread function of the optical beams based on the second signal to identify and correct the optical aberrations within the sample.