US 12,247,993 B2
Specimen platelet aggregation measurement method and specimen platelet aggregation measurement apparatus
Hiroshi Kurono, Kobe (JP); Tasuku Sakayori, Kobe (JP); and Yuri Watanabe, Kobe (JP)
Assigned to SYSMEX CORPORATION, Kobe (JP)
Filed by SYSMEX CORPORATION, Kobe (JP)
Filed on May 18, 2020, as Appl. No. 16/876,491.
Claims priority of application No. 2019-112806 (JP), filed on Jun. 18, 2019.
Prior Publication US 2020/0400702 A1, Dec. 24, 2020
Int. Cl. G01N 35/10 (2006.01); G01N 15/14 (2006.01); G01N 33/487 (2006.01); G01N 15/01 (2024.01)
CPC G01N 35/1016 (2013.01) [G01N 15/14 (2013.01); G01N 33/487 (2013.01); G01N 2015/018 (2024.01); G01N 2035/1032 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A specimen measurement method for measuring platelet aggregation of a specimen, the specimen measurement method comprising:
providing a specimen obtained by centrifuging blood;
displaying a plurality of preset combinations on a display, wherein each preset combination includes a kind of a platelet-aggregating reagent and a predetermined concentration of the platelet-aggregating reagent;
receiving, by a controller, a selection of at least one preset combination of the plurality of preset combinations from among the plurality of preset combinations displayed on the display;
receiving, by the controller via an input box displayed on the display, a test number that:
corresponds to the received at least one preset combination of the kind of the platelet-aggregating reagent and the predetermined concentration of the platelet-aggregating reagent;
represents a number of times a measurement sample will be prepared and measured under a same condition;
calculating a required amount of diluted reagent solution for the received at least one present combination using the received test number as a variable in a linear equation;
automatically preparing a diluted reagent solution that contains a platelet-aggregating reagent diluted with a diluent based on the received at least one preset combination and the received test number, wherein the automatically preparing the diluted reagent solution comprises suctioning the diluent by a dispensing suction tube of a dispenser, suctioning the platelet-aggregating reagent by the dispensing suction tube of the dispenser, and dispensing the diluent and the platelet-aggregating reagent from the dispenser into a diluted reagent solution container for storing the diluted reagent solution;
preparing the measurement sample that contains a predetermined concentration of the platelet-aggregating reagent, from the diluted reagent solution and the specimen; and
performing optical measurement of the measurement sample.
 
19. A specimen measurement apparatus for measuring platelet aggregation of a specimen, the specimen measurement apparatus comprising:
a controller configured to store a plurality of preset combinations, wherein each preset combination includes a kind of a platelet-aggregating reagent and a predetermined concentration of the platelet-aggregating reagent, wherein the controller is configured to receive via user input a selection of at least one preset combination of the plurality of present combinations and a selection of a test number that: a) corresponds to the received at least one preset combination of the kind of the platelet-aggregating reagent and the predetermined concentration of the platelet-aggregating reagent; b) represents a number of times a measurement sample is-will be prepared and measured under a same condition, wherein the controller calculates a required amount of diluted reagent solution for the received at least one preset combination using the received test number as a variable in a linear equation;
an input unit coupled to the controller, the input unit configured to receive input from a user and transmit the input to the controller;
a display coupled to the controller for displaying the plurality of preset combinations;
a diluted reagent solution preparation unit configured to prepare a diluted reagent solution that contains a platelet-aggregating reagent diluted with a diluent based on the received at least one preset combination of the plurality of preset combinations and the received test number, wherein the diluted reagent solution preparation unit is configured to suction the diluent by a dispensing suction tube of a dispenser, and suction the platelet-aggregating reagent by the dispensing suction tube of the dispenser, and dispense the diluent and the platelet-aggregating reagent from the dispenser into a diluted reagent solution container for storing the diluted reagent solution;
a measurement sample preparation unit configured to prepare the measurement sample that contains a predetermined concentration of the platelet-aggregating reagent, from the diluted reagent solution and the specimen; and
a measurement unit configured to perform optical measurement of the measurement sample,
wherein the specimen is obtained by centrifuging blood.