US 12,247,983 B2
Protein A chromatography-electrospray ionization mass spectrometer
Shunhai Wang, Scarsdale, NY (US)
Assigned to Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed by Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed on Jan. 24, 2020, as Appl. No. 16/751,706.
Claims priority of provisional application 62/796,820, filed on Jan. 25, 2019.
Prior Publication US 2020/0240999 A1, Jul. 30, 2020
Int. Cl. G01N 33/68 (2006.01); G01N 30/14 (2006.01); G01N 30/72 (2006.01); H01J 49/16 (2006.01)
CPC G01N 33/6848 (2013.01) [G01N 30/14 (2013.01); G01N 30/7206 (2013.01); G01N 30/7233 (2013.01); G01N 30/7266 (2013.01); G01N 33/6854 (2013.01); H01J 49/165 (2013.01); G01N 2560/00 (2013.01)] 12 Claims
OG exemplary drawing
 
1. A method for identifying at least one protein, said method comprising:
contacting a sample including the protein to a chromatographic system having a protein A chromatography resin;
washing said protein A chromatography resin using a mobile phase to provide an eluate including the protein, wherein said eluate has a more acidic pH after washing; and
identifying the protein in said eluate using an electrospray ionization mass spectrometer run under native conditions,
wherein at least one splitter with at least three paths is used to couple said chromatographic system with said mass spectrometer and with an ultraviolet detector.