| CPC G01N 33/57488 (2013.01) [G01N 33/92 (2013.01); G01N 2405/02 (2013.01); G01N 2405/04 (2013.01); G01N 2405/08 (2013.01)] | 16 Claims |
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1. A method of diagnosing cancer, selected from the group consisting of kidney, breast, and pancreatic cancer, based on lipidomic analysis of a first sample of a body fluid selected from serum, plasma, blood, urine, said first sample of the body fluid being taken from the body of a patient, characterized in that said method comprises the steps of:
spiking of the first sample with a set of internal standards comprising
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subsequently processing the first sample by liquid-liquid lipidomic extraction or by solid phase lipidomic extraction,
measurement of the processed first sample by a mass spectrometry method,
determining concentrations for all lipids present at a level above a detection threshold of the mass spectrometry method, using the internal standards for the corresponding lipid classes, to which a corresponding one of the internal standards belong, for quantitation, or using relative concentrations, or using ratios of concentrations and/or signal intensities,
computer-implemented statistical evaluation of the determined concentrations of the lipids, wherein the statistical evaluation involves principal component analysis (PCA) and/or by orthogonal partial least squares-discriminant analysis (OPLS-DA), said statistical evaluation determining the level of probability of the patient suffering from kidney, breast or pancreatic cancer based on the determined lipid concentrations compared to a kidney, breast or pancreatic cancerous pattern, and a non-cancerous pattern, wherein the kidney, breast or pancreatic cancerous pattern and the non-cancerous pattern are determined by statistical analysis of the lipid concentrations for a group of known cancer samples and non-cancer samples, and wherein the statistical evaluation is done separately for males and females.
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