US 12,247,979 B2
Compositions and methods for capturing exosomes
Gale W. Newman, Duluth, GA (US); Samuel Anyanwu, Atlanta, GA (US); and James W. Lillard, Jr., Smyrna, GA (US)
Filed by Morehouse School of Medicine, Atlanta, GA (US)
Filed on Feb. 21, 2023, as Appl. No. 18/172,059.
Application 18/172,059 is a continuation of application No. 16/566,273, filed on Sep. 10, 2019, abandoned.
Application 16/566,273 is a continuation of application No. 14/702,372, filed on May 1, 2015, abandoned.
Prior Publication US 2023/0341401 A1, Oct. 26, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/573 (2006.01); C07K 16/40 (2006.01); G01N 33/68 (2006.01)
CPC G01N 33/573 (2013.01) [C07K 16/40 (2013.01); G01N 33/6893 (2013.01); C07K 2317/34 (2013.01); G01N 2333/91102 (2013.01)] 12 Claims
 
1. A method for diagnosing a disease or a condition in a subject, comprising:
(a) contacting a bodily fluid sample from the subject with rabbit anti-human ALG6 polyclonal antibodies under conditions suitable for forming complexes comprising exosomes bound to the polyclonal antibodies,
(b) contacting the complexes with at least one disease- or condition-associated biomarker binding agent suitable for detecting at least one disease- or condition-associated biomarkers present in the exosomes; and
(c) determining whether the exosomes comprise at least one detectable disease- or condition-associated biomarker,
wherein detection of the at least one disease- or condition-associated biomarker in step (c) is indicative of a disease or condition in the subject and wherein lack of detection of the at least one disease- or condition-associated biomarker in step (c) is indicative of the absence of the disease or condition in the subject,
wherein the rabbit anti-human ALG6 polyclonal antibodies are made by administering an Ac-YEAQRHWQEIT (SEQ ID NO:1)-Ahx-C synthetic peptide conjugate to a rabbit and purifying the resultant antibodies.