| CPC G01N 33/54388 (2021.08) | 22 Claims |

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1. A system, comprising:
a) a sample concentration device comprising a substrate comprising a plurality of wicking regions and a plurality of capture regions, wherein each of said capture regions comprises a plurality of analyte specific capture reagents, wherein each of said capture regions comprises two fluid isolation zones on either side of said capture region, wherein said fluid isolation zones are physically separatable from said capture region;
b) a detection membrane that receives concentrated sample from a capture region via a primary flow path; and
c) a siphon pad in operable communication with said sample concentration device and said detection membrane, wherein said siphon pad that comprises one or more arms that direct flow from said sample concentration device down a path different than the primary flow path, and wherein the siphon pad does not comprise analyte specific capture reagents.
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