US 12,247,972 B2
Method for measuring intracellular potential with a capacitance type potential measurement device
Mitsuyoshi Saito, Tokyo (JP)
Assigned to ION CHAT RESEARCH CORPORATE, Saitama (JP)
Appl. No. 17/050,759
Filed by ION CHAT RESEARCH CORPORATE, Saitama (JP)
PCT Filed Apr. 26, 2019, PCT No. PCT/JP2019/018158
§ 371(c)(1), (2) Date Oct. 26, 2020,
PCT Pub. No. WO2019/208828, PCT Pub. Date Oct. 31, 2019.
Claims priority of application No. 2018-087689 (JP), filed on Apr. 27, 2018.
Prior Publication US 2021/0231637 A1, Jul. 29, 2021
Int. Cl. G01N 33/483 (2006.01); G01N 27/22 (2006.01); G01N 27/30 (2006.01); G01N 33/15 (2006.01); G01N 33/50 (2006.01); G01N 33/543 (2006.01)
CPC G01N 33/483 (2013.01) [G01N 27/226 (2013.01); G01N 33/15 (2013.01); G01N 33/5058 (2013.01); G01N 33/5061 (2013.01); G01N 27/30 (2013.01); G01N 33/54346 (2013.01)] 5 Claims
OG exemplary drawing
 
1. A capacitance-type potential measurement device capable of recording intracellular potential or potential change of target cells, comprising a first conductor, a second conductor, a magnet, and a charge amplifier,
wherein the first conductor and the second conductor form a capacitor,
wherein the device forms a potential recording circuit by connecting the first conductor to a positive input of the charge amplifier and by connecting the second conductor to a negative input of the charge amplifier,
wherein the first conductor comprises a conductive plate and conductive magnetic nanoparticles,
wherein the conductive plate is disposable below the target cells,
wherein an upper surface of the conductive plate is in contact with an end of the conductive magnetic nanoparticles penetrating a lower surface of the cell membrane in the target cells;
wherein the magnet is placed below the conductive plate, and
wherein the second conductor is a conductive sheet placed between the conductive plate and the magnet.