US 12,247,255 B2
Capture probes and uses thereof
Gabriela Ecco, Saint-Sulpice (CH); Xiaobin Xing, Saint-Sulpice (CH); Adrian Willig, Saint-Sulpice (CH); and Zhenyu Xu, Saint-Sulpice (CH)
Assigned to SOPHIA GENETICS S.A., Rolle (CH)
Filed by Sophia Genetics S.A., Saint-Sulpice (CH)
Filed on Sep. 24, 2021, as Appl. No. 17/484,928.
Claims priority of application No. 21175123 (EP), filed on May 20, 2021.
Prior Publication US 2022/0380843 A1, Dec. 1, 2022
Int. Cl. C12Q 1/6874 (2018.01); C12N 15/10 (2006.01); C12Q 1/6853 (2018.01)
CPC C12Q 1/6874 (2013.01) [C12N 15/1068 (2013.01); C12Q 1/6853 (2013.01)] 8 Claims
 
1. A probe set comprising:
at least one probe comprising:
a first portion of the probe complementary to a primary nucleotide portion of a target nucleic acid, and
a second portion of the probe complementary to a secondary nucleotide portion of the target nucleic acid,
wherein the probe overlaps a breakpoint between the primary and the secondary nucleotide portion of the target nucleic acid, and
wherein the length of second portion of the probe represents about 9% to about 42% of a total probe length, and
at least one further probe complementary only to the primary nucleotide portion of the target nucleic acid, wherein the further probe does not overlap a breakpoint between the primary and the secondary nucleotide portion of the target nucleic acid.