	US 12,247,254 B2
	Compositions and methods for improving sample identification in indexed nucleic acid libraries
Eric Hans Vermaas, San Diego, CA (US); Mahdieh Khosroheidari, San Diego, CA (US); and Claire Bevis-Mott, Cambridge (GB)
Assigned to Illumina, Inc., San Diego, CA (US); and Illumina Cambridge Limited, Cambridge (GB)
Filed by Illumina, Inc., San Diego, CA (US); and Illumina Cambridge Limited, Cambridge (GB)
Filed on Feb. 22, 2021, as Appl. No. 17/181,271.
Application 17/181,271 is a division of application No. 15/959,984, filed on Apr. 23, 2018, granted, now 10,934,584.
Claims priority of provisional application 62/488,833, filed on Apr. 23, 2017.
Prior Publication US 2021/0172014 A1, Jun. 10, 2021
Int. Cl. C12Q 1/6874 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6809 (2018.01); C12Q 1/6816 (2018.01); C12Q 1/6834 (2018.01); C12Q 1/6855 (2018.01); C40B 20/04 (2006.01); C40B 50/16 (2006.01); C40B 50/18 (2006.01); C40B 70/00 (2006.01); C40B 80/00 (2006.01)

CPC C12Q 1/6874 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/6809 (2013.01); C12Q 1/6816 (2013.01); C12Q 1/6834 (2013.01); C12Q 1/6855 (2013.01); C40B 20/04 (2013.01); C40B 50/16 (2013.01); C40B 50/18 (2013.01); C40B 70/00 (2013.01); C40B 80/00 (2013.01)]

17 Claims

1. A polynucleotide prepared for sequencing, comprising

an adapter-target-adapter sequence,

wherein the adapter sequence comprises a library specific sequence,

wherein the polynucleotide comprises a double stranded region,

wherein the 5′ ends are each modified to prevent digestion by an enzyme having 5′ exonuclease activity,

wherein the 3′ ends are each modified to inhibit digestion by an enzyme having 3′ exonuclease activity, to inhibit addition of nucleotides to the 3′ ends by an enzyme having polymerase activity, or to inhibit digestion by an enzyme having 3′ exonuclease activity and to inhibit addition of nucleotides to the 3′ ends by an enzyme having polymerase activity, and

wherein the 3′ ends each comprise a dideoxynucleotide.