US 12,247,253 B2
Methods of sequencing linked fragments
Milenko Despotovic, Richmond (CA); Joel Pel, Vancouver (CA); and Andrea Marziali, North Vancouver (CA)
Assigned to NCAN Genomics, Inc., Vancouver (CA)
Filed by NCAN Genomics, Inc., Vancouver (CA)
Filed on Aug. 1, 2024, as Appl. No. 18/791,664.
Application 18/791,664 is a continuation of application No. 18/489,381, filed on Oct. 18, 2023.
Application 18/489,381 is a continuation of application No. 17/060,853, filed on Oct. 1, 2020, granted, now 11,827,930, issued on Nov. 28, 2023.
Application 17/060,853 is a continuation of application No. 16/008,702, filed on Jun. 14, 2018, granted, now 10,829,813, issued on Nov. 10, 2020.
Application 16/008,702 is a continuation of application No. 14/930,227, filed on Nov. 2, 2015, granted, now 10,000,799, issued on Jun. 19, 2018.
Claims priority of provisional application 62/074,991, filed on Nov. 4, 2014.
Prior Publication US 2024/0392366 A1, Nov. 28, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6869 (2018.01)
CPC C12Q 1/6869 (2013.01) 23 Claims
 
1. A method of preparing a sequencing library, the method comprising:
ligating adapters onto a plurality of nucleic acid fragments, wherein the fragments include a target sequence;
exposing the fragments to a pair of joined primers;
extending the joined primers to produce a first copy of the target sequence linked to a second copy of the target sequence, wherein the linked first and second copies are from sense and anti-sense strands, respectively, of the target sequence and have the same sequence; and
amplifying the linked first and second copies to generate amplification products.