US 12,247,249 B2
Method for amplifying a genomic sample
Samuel Woodhouse, Cambridge (GB); Giovanni Marsico, Cambridge (GB); Vincent Plagnol, Cambridge (GB); and Stefanie Lensing, Cambridge (GB)
Assigned to INIVATA LTD., Cambridge (GB)
Appl. No. 17/311,972
Filed by Inivata Ltd., Cambridge (GB)
PCT Filed Dec. 17, 2019, PCT No. PCT/IB2019/060967
§ 371(c)(1), (2) Date Jun. 8, 2021,
PCT Pub. No. WO2020/128868, PCT Pub. Date Jun. 25, 2020.
Application 17/311,972 is a continuation in part of application No. 16/230,609, filed on Dec. 21, 2018, granted, now 10,533,214.
Prior Publication US 2022/0056508 A1, Feb. 24, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6883 (2018.01); C12Q 1/6827 (2018.01)
CPC C12Q 1/6827 (2013.01) [C12Q 1/6883 (2013.01); C12Q 2600/106 (2013.01); C12Q 2600/156 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A method for amplifying a sample, comprising:
(a) combining a sample of cfDNA from the blood of a human with a thermostable polymerase, dNTPs and a set of at least 10 primers to produce a reaction mix, wherein:
(i) the 3′ end of each primer specifically hybridizes to a sequence that is repeated at least 20 times in the human genome,
(ii) the binding sites for the primers on opposite strands of the human genome have a median interval in the range of 50-114 nucleotides; and
(iii) collectively, the set of primers amplifies at least 50 regions having a total length of at least 100 kb, in a polymerase chain reaction using the human genome as a template; and
(b) subjecting the reaction mix to thermocycling to produce a reaction product comprising at least 50 amplicons having a total length of at least 100 kb.