US 12,247,245 B2
Selectivity screening for antimicrobial compounds
Kim Lewis, Newton, MA (US); Anthony D'Onofrio, Northborough, MA (US); Thomas Curtis, Boston, MA (US); and Charlotte Berkes, Melrose, MA (US)
Assigned to Northeastern University, Boston, MA (US)
Appl. No. 17/262,031
Filed by NORTHEASTERN UNIVERSITY, Boston, MA (US)
PCT Filed May 24, 2019, PCT No. PCT/US2019/033885
§ 371(c)(1), (2) Date Jan. 21, 2021,
PCT Pub. No. WO2020/023106, PCT Pub. Date Jan. 30, 2020.
Claims priority of provisional application 62/703,139, filed on Jul. 25, 2018.
Prior Publication US 2021/0301316 A1, Sep. 30, 2021
Int. Cl. C12Q 1/18 (2006.01)
CPC C12Q 1/18 (2013.01) 20 Claims
 
1. A method of identifying a producer microorganism that makes a compound having selective inhibitory activity against a target microorganism, the method comprising the steps of:
suspending first and second microorganisms in a nutrient medium, wherein (i) the first and second microorganisms are different from each other, (ii) the first microorganism exhibits a first detectable label, (iii) the second microorganism exhibits a second detectable label different from the first detectable label, (iv) neither of the labels interferes with detection of the other label, and (v) the second microorganism is the target microorganism;
contacting the first and second microorganisms with a producer microorganism, that makes the compound, to produce a mixed culture;
incubating the mixed culture;
isolating a volume of the nutrient medium exhibiting the first label but not the second label; and
identifying the producer of the compound having selective inhibitory activity against the target microorganism from said volume.