	US 12,247,242 B2
	Method for quantitatively controlling plasmid copy number in antibiotic-free plasmid maintenance system
Gyoo Yeol Jung, Gyeongsangbuk-do (KR); Sang Woo Seo, Seoul (KR); Chae Won Kang, Daegu (KR); and Hyun Gyu Lim, Chungcheongbuk-do (KR)
Assigned to SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION, Seoul (KR); and POSTECH ACADEMY-INDUSTRY FOUNDATION, Pohang-si (KR)
Appl. No. 16/500,637
Filed by POSTECH ACADEMY-INDUSTRY FOUNDATION, Gyeongsangbuk-do (KR); and SEOUL NATIONAL UNIVERSITY R & DB FOUNDATION, Seoul (KR)
PCT Filed Apr. 5, 2018, PCT No. PCT/KR2018/004038 § 371(c)(1), (2) Date Oct. 19, 2023, PCT Pub. No. WO2018/186704, PCT Pub. Date Oct. 11, 2018.
Claims priority of application No. 10-2017-0044979 (KR), filed on Apr. 6, 2017.
Prior Publication US 2024/0200108 A1, Jun. 20, 2024
Int. Cl. C12N 15/70 (2006.01); C12P 5/02 (2006.01); C12P 7/46 (2006.01)

CPC C12P 7/46 (2013.01) [C12N 15/70 (2013.01); C12P 5/026 (2013.01); C12N 2800/101 (2013.01); C12N 2830/36 (2013.01); C12N 2840/10 (2013.01)]

18 Claims

1. A gene expression cassette containing a synthetic 5′ UTR (untranslated region), a promoter, and a regulatory gene, wherein the synthetic 5′ UTR includes one 5′ UTR selected from a group consisting of SEQ ID NOs: 54, 55, 56 and 59.