US 12,247,240 B2
Recombinant microorganism having increased ability to produce isopropanol and method of producing isopropanol using same
Sung Ok Han, Seoul (KR); Young Jin Ko, Chuncheon-si (KR); and Joy Cha, Seoul (KR)
Assigned to Korea University Research and Business Foundation, Seoul (KR)
Filed by Korea University Research and Business Foundation, Seoul (KR)
Filed on Dec. 7, 2022, as Appl. No. 18/062,834.
Claims priority of application No. 10-2021-0185265 (KR), filed on Dec. 22, 2021.
Prior Publication US 2023/0272436 A1, Aug. 31, 2023
Int. Cl. C12P 7/04 (2006.01); C12N 15/77 (2006.01)
CPC C12P 7/04 (2013.01) [C12N 15/77 (2013.01)] 7 Claims
 
5. A recombinant microorganism for isopropanol production into which a first recombinant vector for isopropanol production comprising a first expression cassette and a second recombinant vector for isopropanol production comprising a second expression cassette are introduced,
wherein the first expression cassette comprises:
a thlA gene consisting of SEQ ID NO: 1;
an atoD gene consisting of SEQ ID NO: 2;
an atoA gene consisting of SEQ ID NO: 3;
an adc gene consisting of SEQ ID NO: 4; and
a sadh gene consisting of SEQ ID NO: 5, and
wherein the second expression cassette comprises:
a phaA gene consisting of SEQ ID NO: 6;
an oxctA gene consisting of SEQ ID NO: 7; and
an oxctB gene consisting of SEQ ID NO: 8.