US 12,247,234 B2
Biocatalysts and methods for hydroxylation of chemical compounds
Haibin Chen, Beijing (CN); Yong Koy Bong, Singapore (SG); Fabien L. Cabirol, Dusseldorf (DE); Anupam Gohel Prafulchandra, Bekasi (ID); Tao Li, Cincinnati, OH (US); Jeffrey C. Moore, Westfield, NJ (US); Martina Quintanar-Audelo, Edinburgh (GB); Yang Hong, Singapore (SG); Steven J. Collier, Concord, MA (US); and Derek Smith, Singapore (SG)
Assigned to Codexis, Inc., Redwood City, CA (US)
Filed by Codexis, Inc., Redwood City, CA (US)
Filed on Nov. 29, 2022, as Appl. No. 18/059,949.
Application 15/357,668 is a division of application No. 14/399,034, granted, now 9,790,527, issued on Oct. 17, 2017, previously published as PCT/US2013/039874, filed on May 7, 2013.
Application 18/059,949 is a continuation of application No. 17/221,613, filed on Apr. 2, 2021, granted, now 11,549,132.
Application 17/221,613 is a continuation of application No. 16/913,761, filed on Jun. 26, 2020, granted, now 10,995,349, issued on May 4, 2021.
Application 16/913,761 is a continuation of application No. 16/446,866, filed on Jun. 20, 2019, granted, now 10,731,189, issued on Aug. 4, 2020.
Application 16/446,866 is a continuation of application No. 15/357,668, filed on Nov. 21, 2016, granted, now 10,370,688, issued on Aug. 6, 2019.
Claims priority of provisional application 61/644,135, filed on May 8, 2012.
Prior Publication US 2023/0227875 A1, Jul. 20, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 17/12 (2006.01); C12N 9/02 (2006.01); C12P 13/04 (2006.01); C12P 13/24 (2006.01); C12P 17/18 (2006.01)
CPC C12P 17/12 (2013.01) [C12N 9/0071 (2013.01); C12P 13/04 (2013.01); C12P 13/24 (2013.01); C12P 17/188 (2013.01); C12Y 114/11002 (2013.01); Y02P 20/52 (2015.11)] 12 Claims
 
1. An engineered polypeptide having proline hydroxylase activity, comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:2 and having a residue difference at residue position X25.